abstract：:Maturation of MC3T3-E1 osteoblast cells in vitro can be divided into three major stages, namely, proliferation, differentiation, and mineralization. Our microarray analysis identified genes differentially expressed between proliferating and differentiated MC3T3-E1 osteoblastic cells. Immunohistochemical analyses of RN...

journal_title：Experimental cell research

authors： Gao Y,Ganss BW,Wang H,Kitching RE,Seth A

更新日期：2005-03-10 00:00:00

abstract：:CIN85 is a multidomain protein that associates with receptors carrying tyrosine kinase domains. Here we report that it is also a component of the signaling complex associated with tumor necrosis factor receptor 1 (TNFR1), which lacks a tyrosine kinase domain. This was established by showing that CIN85 was co-precipita...

journal_title：Experimental cell research

authors： Narita T,Nishimura T,Yoshizaki K,Taniyama T

更新日期：2005-03-10 00:00:00

abstract：:Differentiation of endoderm into intestinal epithelium is initiated at E13.5 of mouse development when there are significant changes in morphology resulting in the conversion of undifferentiated stratified epithelium into a mature epithelial monolayer. Here we demonstrate that monolayer formation is associated with th...

journal_title：Experimental cell research

authors： Abud HE,Watson N,Heath JK

更新日期：2005-02-15 00:00:00

abstract：:We have reported that PAP-1, a product of a causative gene for autosomal retinitis pigmentosa, plays a role in splicing. In this study, CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor, was identified as a PAP-1 binding protein and its function as ...

journal_title：Experimental cell research

authors： Maita H,Kitaura H,Ariga H,Iguchi-Ariga SM

更新日期：2005-02-15 00:00:00

abstract：:Laminins are structurally and functionally major components of the extracellular matrix. Four isoforms of laminins (laminin-1, -2, -5 and -10) are expressed in a specific pattern along the crypt-villus axis of the intestine. Previous works indicated that expression of these isoforms is developmentally regulated and th...

journal_title：Experimental cell research

authors： Turck N,Gross I,Gendry P,Stutzmann J,Freund JN,Kedinger M,Simon-Assmann P,Launay JF

更新日期：2005-02-15 00:00:00

abstract：:The Cdc25 phosphatases play crucial roles in cell cycle progression by removing inhibitory phosphates from tyrosine and threonine residues of cyclin-dependent kinases. Cdc25A is an important regulator of the G1/S transition but functions also in the mitotic phase of the human cell cycle. In this paper, we investigate ...

journal_title：Experimental cell research

authors： Källström H,Lindqvist A,Pospisil V,Lundgren A,Rosenthal CK

更新日期：2005-02-01 00:00:00

abstract：:RNA polymerase II transcripts are confined to nuclear compartments. A detailed analysis of the nuclear topology of RNA from individual genes was performed for transcripts from the marker gene coding for chloramphenicol acetyltransferase, expressed at a high level from the HTLV-1 LTR promoter. The construct was transfe...

journal_title：Experimental cell research

authors： Bridger JM,Kalla C,Wodrich H,Weitz S,King JA,Khazaie K,Kräusslich HG,Lichter P

更新日期：2005-01-15 00:00:00

abstract：:USF is a small family of basic helix-loop-helix leucine zipper (bHLH-zip) transcription factors with DNA binding specificities similar to that of the c-Myc oncoprotein. Evidence for a role of USF in growth control includes inhibition of c-Myc-dependent cellular transformation in vitro and loss of USF transcriptional a...

journal_title：Experimental cell research

authors： Choe C,Chen N,Sawadogo M

更新日期：2005-01-01 00:00:00

abstract：:The epidermal growth factor receptor (EGFR) is fundamental for normal cell growth and organ development, but has also been implicated in various pathologies, notably tumors of epithelial origin. We have previously shown that the initial 13 amino acids (P13) within the intracellular juxtamembrane region (R645-R657) are...

journal_title：Experimental cell research

authors： Aifa S,Aydin J,Nordvall G,Lundström I,Svensson SP,Hermanson O

更新日期：2005-01-01 00:00:00

abstract：:The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...

journal_title：Experimental cell research

authors： Lolkema MP,Mehra N,Jorna AS,van Beest M,Giles RH,Voest EE

更新日期：2004-12-10 00:00:00

abstract：:Nucleotide sugar transporters play a central role in the process of glycosylation. They are responsible for the translocation of nucleotide sugars from the cytosol, their site of synthesis, into the Golgi apparatus where the activated sugars serve as substrates for a variety of glycosyltransferases. We and others have...

journal_title：Experimental cell research

authors： Lühn K,Laskowska A,Pielage J,Klämbt C,Ipe U,Vestweber D,Wild MK

更新日期：2004-12-10 00:00:00

abstract：:Taking the advantage of single-molecule imaging, our recent study has revealed surprisingly long processive movement of a Formin protein, mDia1, surfing along with the growing end of actin filaments in living cells. This finding provides direct evidence for the ability of Formins to function as processive cappers that...

journal_title：Experimental cell research

authors： Watanabe N,Higashida C

更新日期：2004-11-15 00:00:00

abstract：:Intermediate filaments (IFs), desmosomes, and their associates are built from multidomain proteins that form cytoskeletal scaffolds in the cytoplasm and the nucleus of vertebrate tissues. Mutations in more than 80 genes cause monogenic disorders that include severe skin fragility, myopathies, neurodegeneration, and pr...

journal_title：Experimental cell research

authors： Magin TM,Reichelt J,Hatzfeld M

更新日期：2004-11-15 00:00:00

abstract：:Lithium exerts neuroprotective actions that involve the inhibition of glycogen synthase kinase-3beta (GSK-3beta). Otherwise, recent studies suggest that sustained GSK-3beta inhibition is a hallmark of tumorigenesis. In this context, the present study was undertaken to examine whether lithium modulated cancer cell sens...

journal_title：Experimental cell research

authors： Beurel E,Kornprobst M,Blivet-Van Eggelpoël MJ,Ruiz-Ruiz C,Cadoret A,Capeau J,Desbois-Mouthon C

更新日期：2004-11-01 00:00:00

abstract：:This study was designed to elucidate the mechanisms involved in elevated cell death arising from an altered endogenous oxidant state. Increased levels of cell death were detected in cells lacking Gpx1 following the addition of exogenous H2O2. This increased apoptosis correlated with a down-regulation in the activation...

journal_title：Experimental cell research

authors： Taylor JM,Crack PJ,Gould JA,Ali U,Hertzog PJ,Iannello RC

更新日期：2004-11-01 00:00:00

abstract：:Microsomal triglyceride transfer protein (MTP) is a heterodimeric complex consisting of a unique large 97-kDa protein and the multifunctional 58-kDa protein disulfide isomerase (PDI). It plays an essential role in the assembly of lipoproteins by shuttling lipids between phospholipid membranes. Based on cell fractionat...

journal_title：Experimental cell research

authors： Slight I,Bendayan M,Malo C,Delvin E,Lambert M,Levy E

更新日期：2004-10-15 00:00:00

abstract：:Endothelial cell-selective adhesion molecule (ESAM) is an immunoglobulin-like transmembrane protein associated with endothelial tight junctions (TJ). Based on a yeast two-hybrid screen, we have identified the membrane-associated guanylate kinase protein MAGI-1 as an intracellular binding partner of ESAM. MAGI-1 is a m...

journal_title：Experimental cell research

authors： Wegmann F,Ebnet K,Du Pasquier L,Vestweber D,Butz S

更新日期：2004-10-15 00:00:00

abstract：:Syndecan-1 was overexpressed in T47D, MCF-7, or Hs578t human breast carcinoma cell lines, mimicking overexpression observed in carcinomas in vivo. Overexpression of syndecan-1, or its ectodomain alone fused to a glycosylphosphatidylinositol anchor (GPI-mS1ED), promotes cell rounding in 2D culture. Deletions within the...

journal_title：Experimental cell research

authors： Burbach BJ,Ji Y,Rapraeger AC

更新日期：2004-10-15 00:00:00

abstract：:We have shown previously that the activity of the long myosin light chain kinase (MLCK) is cell cycle regulated with a decrease in specific activity during mitosis that can be restored following treatment with alkaline phosphatase. To better understand the role and significance of phosphorylation in regulating MLCK fu...

journal_title：Experimental cell research

authors： Dulyaninova NG,Bresnick AR

更新日期：2004-10-01 00:00:00

abstract：:The periderm is an epithelial layer covering the emerging epidermis in early embryogenesis of vertebrates. In the chicken embryo, an additional cellular layer, the subperiderm, occurs at later embryonic stages underneath the periderm. The questions arose what is the function of both epithelial layers and, as they are ...

journal_title：Experimental cell research

authors： Saathoff M,Blum B,Quast T,Kirfel G,Herzog V

更新日期：2004-10-01 00:00:00

abstract：:Bcl-2 family proteins regulate apoptosis at the level of mitochondria. To examine the mechanism of Bcl-2 function, we investigated the effects of the protonophore carbonyl cyanide m-chlorophenyl hydrazone (CCCP) on two hematopoietic cell lines and Bcl-2 overexpressing transfectants. CCCP directly interferes with mitoc...

journal_title：Experimental cell research

authors： de Graaf AO,van den Heuvel LP,Dijkman HB,de Abreu RA,Birkenkamp KU,de Witte T,van der Reijden BA,Smeitink JA,Jansen JH

更新日期：2004-10-01 00:00:00

abstract：:The aim of the present study was to determine whether mitochondrial activity could regulate myoblast proliferation. We demonstrate that an increase in mitochondrial activity of L6E9 myoblasts can be easily obtained by simply raising extracellular pyruvate concentration in the culture dish. Under this condition, L6E9 m...

journal_title：Experimental cell research

authors： Duguez S,Sabido O,Freyssenet D

更新日期：2004-09-10 00:00:00

abstract：:The bone-related transcription factor Runx2 (Cbfa1) has been extensively shown to regulate osteoblast differentiation and function. Recent studies demonstrate that Runx2 is also a positive regulator of chondrocyte maturation and vascular invasion in cartilage. Runx2 activity can be modulated in several ways, including...

journal_title：Experimental cell research

authors： Li TF,Dong Y,Ionescu AM,Rosier RN,Zuscik MJ,Schwarz EM,O'Keefe RJ,Drissi H

更新日期：2004-09-10 00:00:00

abstract：:Tumor cell adhesion within host organ microvasculature, its stabilization and invasion into host organ parenchyma appear to be important steps during formation of distant metastasis. These interactions of circulating tumor cells with the host organs occur in the presence of fluid shear forces and soluble and cellular ...

journal_title：Experimental cell research

authors： Korb T,Schlüter K,Enns A,Spiegel HU,Senninger N,Nicolson GL,Haier J

更新日期：2004-09-10 00:00:00

abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...

journal_title：Experimental cell research

authors： Kinnaird JH,Maitland K,Walker GA,Wheatley I,Thompson FJ,Devaney E

更新日期：2004-08-15 00:00:00

abstract：:Dynamic subcellular localization is an important regulatory mechanism for many proteins. cIAP1 and cIAP2 are two closely related members of inhibitor of apoptosis (IAP) family that play a role both as caspase inhibitors and as mediators of tumor necrosis factor (TNF) receptor signaling. Here, we report that cIAP1 and ...

journal_title：Experimental cell research

authors： Vischioni B,Giaccone G,Span SW,Kruyt FA,Rodriguez JA

更新日期：2004-08-15 00:00:00

abstract：:Abrupt developmental changes occur in structural form and function of connexin (Cx) channels in the mouse mammary gland. Microarray study shows that the principal connexin isoform in epithelial cells during pregnancy is Cx26, up-regulated and persisting from the virgin. After parturition, there is rapid induction of C...

journal_title：Experimental cell research

authors： Locke D,Stein T,Davies C,Morris J,Harris AL,Evans WH,Monaghan P,Gusterson B

更新日期：2004-08-15 00:00:00

abstract：:We report that prosaposin binds to U937 and is active as a protective factor on tumor necrosis factor alpha (TNFalpha)-induced cell death. The prosaposin-derived saposin C binds to U937 cells in a concentration-dependent manner, suggesting that prosaposin behaves similarly. Prosaposin binding induces U937 cell death p...

journal_title：Experimental cell research

authors： Misasi R,Garofalo T,Di Marzio L,Mattei V,Gizzi C,Hiraiwa M,Pavan A,Grazia Cifone M,Sorice M

更新日期：2004-08-01 00:00:00

abstract：:Primary erythroid cells and erythroid cell lines may synthesize and secrete tumor necrosis factor-alpha (TNF-alpha) following stimulation with erythropoietin (EPO). The effect of triggering TNF-alpha synthesis and secretion was investigated in erythroleukemia and myeloid cell lines: HCD57, DA3-EPOR, and BAF3-EPOR. The...

journal_title：Experimental cell research

authors： Chen J,Jacobs-Helber SM,Barber DL,Sawyer ST

更新日期：2004-08-01 00:00:00

abstract：:The epigenetic states of key regulatory genes must be altered to drive cell fate decisions in differentiating cells. This process must be coupled, at least transiently, to the DNA replication machinery. Only a few genes, however, have been shown to require DNA replication for their activation or repression upon induct...

journal_title：Experimental cell research

authors： Bancescu DL,Glatt-Deeley H,Lalande M

更新日期：2004-08-01 00:00:00

abstract：:Dystrophin is a cytoskeletal protein normally expressed underneath the sarcolemma of muscle fibers. The lack of dystrophin in Duchenne muscular Dystrophy (DMD) muscles results in fiber necrosis, which was proposed to be mediated by chronic calcium mishandling. The extensive comparison of dystrophic cells from human or...

journal_title：Experimental cell research

authors： Marchand E,Constantin B,Balghi H,Claudepierre MC,Cantereau A,Magaud C,Mouzou A,Raymond G,Braun S,Cognard C

更新日期：2004-07-15 00:00:00

abstract：:The glial cell line-derived neurotrophic factor (GDNF) is involved in the development and maintenance of neural tissues. Mutations in components of its signaling pathway lead to severe migration deficits of neuronal crest stem cells, tumor formation, or ablation of the urinary system. In animal models of Parkinson's d...

journal_title：Experimental cell research

authors： Pahnke J,Mix E,Knoblich R,Müller J,Zschiesche M,Schubert B,Koczan D,Bauer P,Böttcher T,Thiesen HJ,Lazarov L,Wree A,Rolfs A

更新日期：2004-07-15 00:00:00

abstract：:Cystatins constitute a superfamily of cysteine protease inhibitors. A member of the type II secreted cystatin family, cystatin F, has been identified through different gene array experiments to be specifically expressed in hematopoietic cells as well as to be associated with several malignant tumors, suggesting a role...

journal_title：Experimental cell research

authors： Cappello F,Gatti E,Camossetto V,David A,Lelouard H,Pierre P

更新日期：2004-07-15 00:00:00

abstract：:The Nir/rdgB family of proteins has been identified in a variety of eukaryotic organisms, ranging from worms to mammals. The Drosophila retinal degeneration B (rdgB), a protein that is required for photoreceptor cell viability and light response, was the first to be identified. It consists an amino-terminal phosphatid...

journal_title：Experimental cell research

authors： Lev S

更新日期：2004-07-01 00:00:00

abstract：:The c-Myb transcription factor plays a central role in the regulation of cell growth and differentiation of hematopoietic cells. Being the product of a proto-oncogene, one would expect c-Myb function to be modulated by signal transduction pathways, but our knowledge on such regulation of c-Myb is rather limited. Recen...

journal_title：Experimental cell research

authors： Dahle Ø,Bakke O,Gabrielsen OS

更新日期：2004-07-01 00:00:00

abstract：:ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B(1...

journal_title：Experimental cell research

authors： Jaramillo BE,Ponce A,Moreno J,Betanzos A,Huerta M,Lopez-Bayghen E,Gonzalez-Mariscal L

更新日期：2004-07-01 00:00:00

abstract：:Protein kinase B (Akt1) holds a central role for cellular growth, development, and survival, but the cellular pathways of Akt1 that prevent inflammatory demise in the vascular system remain undefined. Employing a constitutively active form of Akt1 (myristoylated Akt1) in endothelial cells (ECs), we demonstrate that Ak...

journal_title：Experimental cell research

authors： Chong ZZ,Kang JQ,Maiese K

更新日期：2004-06-10 00:00:00

abstract：:Procollagen I is a trimer consisting of two proalpha1(I) chains and one proalpha 2(I) chain. In certain cases of mild osteogenesis imperfecta, abnormal proalpha1(I) chains are degraded very soon after synthesis. As a consequence, the cells produce excess proalpha2(I) chains, which cannot form trimers and are not secre...

journal_title：Experimental cell research

authors： Gotkin MG,Ripley CR,Lamande SR,Bateman JF,Bienkowski RS

更新日期：2004-06-10 00:00:00

abstract：:The germinal vesicle (GV) of Xenopus laevis is an enormous nucleus that contains 18 giant lampbrush chromosomes and thousands of inclusions. The inclusions are primarily of three types: approximately 1500 extrachromosomal nucleoli, 50-100 Cajal bodies, and several thousand B-snurposomes, which correspond to speckles o...

journal_title：Experimental cell research

authors： Gall JG,Wu Z,Murphy C,Gao H

更新日期：2004-05-15 00:00:00

abstract：:N-methyl-d-aspartate (NMDA) subtype of glutamate receptors plays an important role during brain development and receptor activation has been shown to promote neuronal survival. The mechanisms by which NMDA increases neuronal survival are not fully understood. Here, we show that treatment with low concentrations of NMD...

journal_title：Experimental cell research

authors： Korhonen L,Näpänkangas U,Steen H,Chen Y,Martinez R,Lindholm D

更新日期：2004-05-01 00:00:00